University of Szeged
Dept. Biochemistry and Molecular Biology

Genomics/sequencing lab

The genomics lab houses an Illumina MiSeq NGS sequencer suitable for various genomic and functional genomic applications. Various auxiliary equipment are also available.

MiSeq bench-top sequencers allow the longest sequencing read lengths (2x300 nucleotides) among the market leading Illumina sequencers. MiSeq supports paired-end sequencing; the maximum output per sequencing run is 15 Gb. Sequencing parameters, such as sequencing depth and read length can be set in wide ranges (1M - 25M sequencing reads per run and 2x50 - 2x300 nucleotides per read, respectively) by selecting suitable flow cells and reagent cartridges.

MiSeq is suitable for several genomic and functional genomic applications, such as:

  • de novo bacterial genome sequencing
  • targeted re-sequencing
  • metagenomics (16S amplicon sequencing)
  • SNP analysis
  • RNA-sequencing / transcriptomics
  • miRNA analysis
  • ChIP-seq

Other auxiliary equipment in the lab:

  • Covaris M220 ultrasonicator
  • Agilent Bioanalyzer 2100 capillary electrophoresis equipment
  • Thermo Scientific PikoReal 96 and ABI 7500 real-time PCR systems
  • Qubit 2.0 fluorometer
  • NanoDrop spectrophotometer
  • SciGene Hybex Microsample Incubator
  • Biosan MPS-1 Multiplate shaker
  • Biosan UVT-B-AR PCR cabinet

In case you are interested in the services of the lab either in the form of research cooperation or as a service, please write to Dr Laszlo Bodai or Dr Imre Boros.

Department of Biochemistry and Molecular Biology, University of Szeged